General Research
Model: rat
Protective Effects of Hydrogen-Rich Saline Against Erectile Dysfunction in a Streptozotocin Induced Diabetic Rat Model
Simplified Version Available
Hydrogen-Rich Saline: A Potential Solution for Erectile Dysfunction in Diabetic Men
A study found that hydrogen-rich saline may help alleviate erectile dysfunction in diabetic men by improving blood flow and reducing oxidative stress. The research used a diabetic rat model and showed promising results. This could be a new hope for men struggling with ED, especially those with diabetes.
Read Simplified ArticleAbstract
Publish Year 2013 Country China Rank Positive Journal Journal of Urology Primary Topic Penis Secondary TopicDiabetes (Type II) Model Rat Tertiary TopicErectile Dysfunction Vehicle Saline (Dissolved) pH Neutral Application Gavage Comparison Complement
Purpose
Materials and methods: Diabetes was induced in Sprague-Dawley® rats by a single intravenous injection of streptozotocin. Diabetic rats were then randomized to a diabetes mellitus group and to a diabetic group that received hydrogen saline. The latter 8 rats were fed saturated hydrogen saline (5 ml/kg per day) by intragastric administration for 8 weeks. At the end of week 8 erectile function was assessed by measuring the increase in intracavernous pressure after cavernous nerve electrostimulation. We measured nitric oxide synthase activity, and malondialdehyde, 8-hydroxydeoxyguanosine, and nitrite and nitrate in the corpus cavernosum. eNOS protein immunolocalization in cavernous tissues was detected by immunohistochemistry. eNOS, Bcl-2 and Bax protein expression was determined by Western blot. We determined eNOS, Bcl-2 and Bax mRNA using real-time reverse transcriptase-polymerase chain reaction.