General Research
Model: human
Molecular hydrogen attenuates fatty acid uptake and lipid accumulation through downregulating CD36 expression in HepG2 cells
Simplified Version Available
How Hydrogen Gas May Help Fight Fat Accumulation and Obesity
A study from Japan found that hydrogen gas can reduce fat accumulation in cells by targeting the CD36 protein. This discovery may lead to new therapies for obesity and related health issues. Further research is needed to fully understand the potential benefits of hydrogen gas for weight management and overall health.
Read Simplified ArticleAbstract
Publish Year 2013 Country Japan Rank Positive Journal Medical Gas Research Primary Topic Whole Body Secondary TopicObesity Model Cell Culture Tertiary TopicLipid Metabolism Vehicle Gas pH N/A Application Ventilation Comparison Complement
Background
Methods: Human hepatoma HepG2 cells were exposed to palmitate-BSA complex after treatment with or without hydrogen for 24 h. The fatty acid uptake was measured by using spectrofluorometry and the lipid content was detected by Oil Red O staining. JNK phosphorylation and CD36 expression were analyzed by Western blot and real-time PCR analyses.
Methods
Results: Pretreatment with hydrogen reduced fatty acid uptake and lipid accumulation after palmitate overload in HepG2 cells, which was associated with inhibition of JNK activation. Hydrogen treatment did not alter CD36 mRNA expression but reduced CD36 protein expression.
Results
Conclusion: Hydrogen inhibits fatty acid uptake and lipid accumulation through the downregulation of CD36 at the protein level in hepatic cultured cells, providing insights into the molecular mechanism underlying the hydrogen effects in vivo on lipid metabolism disorders.