General Research
Model: rat
The effect of hydrogen-rich saline on the brain of rats with transient ischemia
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Hydrogen-Rich Saline: A Potential Brain Savior
Hydrogen-rich saline has been shown to reduce brain damage in rats with transient ischemia, a condition where blood flow to the brain is cut off. This could have big implications for human health, especially for people who have had strokes or other brain injuries. By reducing oxidative stress, hydrogen-rich saline may be a valuable tool in the fight against brain damage.
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Publish Year 2011 Country China Rank Positive Journal Journal of Surgical Research Primary Topic Brain Secondary TopicBrain Injury Model Rat Tertiary TopicIschemia-Reperfusion Injury Vehicle Saline (Dissolved) pH Neutral Application Injection Comparison Complement
Background
Materials and methods: We used a four-vessel occlusion model of global cerebral ischemia (15 min) and reperfusion with rats. The rats were divided into four groups (n = 96): sham, I/R plus physiologic saline injected intraperitoneally, I/R plus hydrogen-rich saline injected intraperitoneally at the beginning of reperfusion, and I/R plus hydrogen-rich saline injected intraperitoneally 6 h after reperfusion began. One group of rats was sacrificed after 24 h of reperfusion. Malondialdehyde (MDA) was measured to quantify the oxidative stress. Caspase-3 was measured to indicate the status of apoptosis. Tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and nuclear factor-κB (NF-κB) were measured to monitor the inflammation. Another group of rats was sacrificed after 72 h of reperfusion to measure the histologic damages in hippocampus by hematoxylin and eosin staining and Nissl staining.