General Research
Model: mouse
Hydrogen-Rich Saline-A Novel Neuroprotective Agent in a Mouse Model of Experimental Cerebral Ischemia via the ROS-NLRP3 Inflammasome Signaling Pathway In Vivo and In Vitro
Simplified Version Available
Hydrogen-Rich Saline: A New Hope for Brain Injury Treatment
A recent study found that hydrogen-rich saline can be a novel neuroprotective agent, helping to protect the brain from damage. The researchers used a mouse model of experimental cerebral ischemia and found that hydrogen-rich saline injection reduced brain damage and improved outcomes. This breakthrough could lead to new treatments for brain injuries and highlights the potential of hydrogen gas as a therapeutic agent.
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Publish Year 2023 Country China Rank Positive Journal Brain Sciences Primary Topic Brain Secondary TopicBrain Injury Model Mouse Tertiary TopicHypoxia-Ischemia Vehicle Saline (Dissolved) pH Neutral Application Injection Comparison Complement
Background
Methods: To test the therapeutic effect of HS, we established a mouse model of distal middle cerebral artery occlusion (dMCAO) and an in vitro model of BV2 cells induced by lipopolysaccharide (LPS). The ROS scavenger N-acetylcysteine (NAC) was used to investigate the underlying mechanisms of HS.
Methods
Results: HS significantly improved neurological function, reduced infarct volume, and increased cerebral blood flow in a dMCAO mouse model. ROS, NLRP3, Caspase-1, and IL-1β expression increased after cerebral ischemia, and this was reversed by HS treatment. In BV2 cells, the application of NAC further demonstrated that HS could effectively inhibit the expression of the ROS-activated NLRP3 inflammasome. Conclusions: HS, as a novel therapeutic option, could exert protect the brain by inhibiting the activation of the ROS-NLRP3 signaling pathway after cerebral ischemia.