Hydrogen Water
General Research Model: rat

Protective Effects of Hydrogen-Rich Saline on Experimental Intestinal Volvulus in Rats

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How Hydrogen-Rich Saline May Help Prevent Intestinal Damage

A study in rats found that hydrogen-rich saline may help prevent intestinal damage caused by intestinal volvulus, a serious condition where the intestine twists and cuts off blood flow. The treatment uses hydrogen gas to neutralize free radicals and promote healing. This discovery could lead to new treatments for intestinal volvulus and other conditions.

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Abstract

Publish Year 2022 Country Turkey Rank Positive Journal Journal of Investigative Surgery Primary Topic Intestine Secondary TopicSurgery/Transplantation Model Rat Tertiary TopicIntestinal Volvulus Vehicle Saline (Dissolved) pH Neutral Application Injection Comparison Complement

Background

Methods: Thirty rats were randomly allocated into 5 groups. All procedures were done under general anesthesia and sterile conditions in each animal. Five ml/kg of saline and HRSS were administered intraperitoneally (ip) in Sham (Group 1) and HRSS (Group 2) groups, respectively. Groups 3, 4, and 5 constituted the study groups in which volvulus was created in a 5-cm- long ileal segment 2 cm proximal to the ileocecal valve. After 2 hours the volvuli were reduced and following 2 hours of reperfusion, these segments were removed. In volvulus-I/R group (Group 3) no additional procedure was done. HRSS was administered shortly before reperfusion (reduction of the volvulus) in Treatment I (Group 4) and 1 h before experimental volvulus in Treatment II (Group 5) groups. Blood and intestinal tissue samples were obtained from all rats at the 4th hour. Both tissue and blood total oxidant (TOS) and antioxidant status (TAS) levels were determined and tissue histomorphologies were studied. Oxidative stress indices (TOS รท TAS) (OSI) were calculated.

Methods

Results: Tissue TOS and OSI levels and histomorphological injury scores were statistically lower in treatment groups than I/R group, whereas blood TOS and OSI levels were similar between the groups. Conclusions: This study provides biochemical and histomorphological evidence that HRSS prevents intestinal damage in I/R injury caused by volvulus.