General Research
Model: human
Protective effects of hydrogen-rich medium on lipopolysaccharides-induced injury in human periodontal ligament cells
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How Hydrogen Can Help Protect Your Gums from Infection and Inflammation
Gum disease is a common problem that can lead to tooth loss if left untreated. Recent research suggests that hydrogen may hold the key to protecting your gums from infection and inflammation. A hydrogen-rich medium has been shown to protect human periodontal ligament cells from injury caused by bacterial toxins, and may be a useful addition to treatment plans for people with gum disease.
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Publish Year 2018 Country China Rank Positive Journal West China Journal of Stomatology Primary Topic Mouth Secondary TopicPeriodontitis Model Cell Culture Tertiary TopicInflammation Vehicle Medium (Dissolved) pH Neutral Application Culture Media Comparison Complement
Methods
Results: CCK-8 results showed that hydrogen could significantly improve hPDLCs growth and decrease cell apoptosis under LPS stimulation (P<0.05). However, no significant difference in LDH release was found between the two groups. The CAT levels significantly increased at 6 and 12 h in the hydrogen-rich medium as compared with the normal medium group (P<0.05, P<0.01, respectively). However, SOD levels were not significant different at each time point. At 6 h after LPS stimulation, the MDA levels in the cell supernatant of hydrogen-rich medium group were significantly reduced as compared with those in the normal medium group (P<0.05). Conclusions: The hydrogen-rich medium can effectively improve hPDLCs proliferation activity and antioxidant capacity and reduce apoptosis and oxidative stress under LPS stimulation.