General Research
Model: human
Molecular hydrogen protects human lymphocyte AHH-1 cells against 12C6+ heavy ion radiation
Simplified Version Available
The Power of Molecular Hydrogen: Protecting Cells from Radiation Damage
Molecular hydrogen has been shown to protect cells from radiation damage, and could have implications for space travel and everyday health. A recent study found that molecular hydrogen protected human lymphocyte AHH-1 cells against heavy ion radiation. This discovery could lead to new ways to protect ourselves from radiation exposure.
Read Simplified ArticleAbstract
Publish Year 2013 Country China Rank Positive Journal International Journal of Radiation Biology Primary Topic Whole Body Secondary TopicSpaceflight Model Cell Culture Tertiary TopicRadioprotection Vehicle Medium (Dissolved) pH Neutral Application Culture Media Comparison Complement
Results
Conclusion: In conclusion, these data showed that H(2) attenuated (12)C(6+) radiation-induced cell apoptosis through reducing the ROS level and modulating apoptotic molecules, thus indicating the potential of H(2) as a safe and effective radioprotectant.
Purpose
Materials and methods: H(2) was dissolved in Roswell Park Memorial Institute (RPMI) 1640 medium under high pressure (0.4 Mpa) to a saturated level by using an apparatus produced by our department. A 2-[6-(4'-hydroxy) phenoxy-3H-xanthen-3-on-9-yl] benzoate (HPF) probe and a 2',7'-Dichlorodihydrofluorescein diacetate (H2DCFH-DA) fluorescent dye were used to measure the intracellular reactive oxygen species (ROS) level. Cell apoptosis were determined by double-staining with Annexin V-fluorescein isothiocyanate (Annexin V-FITC) and propidium iodide (PI) as well as a Hoechst 33342 staining method alternatively. Subsequently, cell cycle analysis was performed using a PI staining method and the expression of apoptotic protein was examined by Western blot.