General Research
Model: mouse
iTRAQ-Based Quantitative Proteomic Analysis of Intestines in Murine Polymicrobial Sepsis with Hydrogen Gas Treatment
Simplified Version Available
How Hydrogen Gas May Help Fight Sepsis and Protect Your Intestines
A recent study found that hydrogen gas treatment may help reduce inflammation and protect the intestines from damage in mice with sepsis. The study's findings have implications for new treatments for sepsis and other conditions that affect the intestines. Hydrogen gas treatment has shown promise in reducing inflammation and improving organ function.
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Publish Year 2020 Country China Rank Positive Journal Drug Design, Development and Therapy Primary Topic Intestine Secondary TopicSepsis Model Mouse Tertiary TopicMultiple Organ Dysfunction Syndrome Vehicle Gas pH N/A Application Inhalation Comparison Complement
Methods
Results: H2 can significantly improve the 7-day survival rates of sepsis mice. The load of blood and peritoneal lavage bacteria was increased, and H2 treatment can significantly reduce it. CLP mice had significant intestinal damage, and inhalation of 2% hydrogen could significantly reduce this damage. All 4194 proteins were quantified, of which 199 differentially expressed proteins were associated with the positive effect of H2 on sepsis. Functional enrichment analysis indicated that H2 may reduce intestinal injury in septic mice through the effects of thyroid hormone synthesis and nitrogen metabolism signaling pathway. Western blot showed that H2 was reduced by down-regulating the expressions of deleted in malignant brain tumors 1 protein (DMBT1), insulin receptor substrate 2 (IRS2), N-myc downregulated gene 1 (NDRG1) and serum amyloid A-1 protein (SAA1) intestinal damage in sepsis mice.
Results
Conclusion: A total of 199 differential proteins were related with H2 in the intestinal protection of sepsis. H2-related differential proteins were notably enriched in the following signaling pathways, including thyroid hormone synthesis signaling pathway, nitrogen metabolism signaling pathways, digestion and absorption signaling pathways (vitamins, proteins and fats). H2 reduced intestinal injury in septic mice by down-regulating the expressions of SAA1, NDRG1, DMBT1 and IRS2.