General Research
Model: mouse
Hydrogen-Rich Water Ameliorates Murine Chronic Graft-versus-Host Disease through Antioxidation
Simplified Version Available
How Hydrogen-Rich Water Can Help with Chronic Graft-Versus-Host Disease
A recent study found that drinking hydrogen-rich water can help reduce the symptoms of chronic graft-versus-host disease, a serious condition that can occur after a bone marrow transplant. The antioxidant properties of hydrogen-rich water may help reduce oxidative stress and improve health. This discovery could lead to a new, simple way to manage cGVHD and potentially other conditions.
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Publish Year 2021 Country China Rank Positive Journal Oxidative Medicine and Cellular Longevity Primary Topic Bone Marrow Secondary TopicSurgery/Transplantation Model Mouse Tertiary TopicGraft-Versus-Host-Disease Vehicle Water (Dissolved) pH Neutral Application Ingestion Comparison Complement
Background
Methods: A mouse cGVHD model was established by haploidentical bone marrow transplantation. To evaluate therapeutic effects of H2 on cGVHD, survival rate, changes in clinical scores, and skin pathologic characteristics of cGVHD mice were observed. To evaluate its therapeutic mechanism, we detected the expression levels of antioxidative enzymes heme oxygenase-1(HO-1) and NAD (P)H: quinone acceptor oxidoreductase 1(NQO1) in skin homogenates. We also detected the expression level of the apoptotic protein caspase-3 in skin homogenates.
Methods
Results: 1-month survival rate of cGVHD mice in the hydrogen group reached 93.3%, significantly higher than 66.7% in the nonhydrogen group (p < 0.05). Clinical score of cGVHD mice was improved by hydrogen-rich water at 96 days posttransplantation (2.2 versus 4.5, p < 0.05). The skin pathological condition of cGVHD mice was significantly improved by hydrogen-rich water. At 96 days posttransplantation, average skin pathological hematoxylin and eosin (HE) staining score in the hydrogen group was 1.05, which was significantly lower than 3.2 in the nonhydrogen group (p < 0.01). Average Masson staining score was 0.6 point in the hydrogen group, lower than 0.9 point in the nonhydrogen group (p < 0.05). Both the relative expression levels of HO-1 and NQO1 proteins in skin specimens of cGVHD mice in the hydrogen group were lower than that in the nonhydrogen group (2.47 versus 6.21 and 1.83 versus 3.59, p < 0.05). The relative expression level of caspase-3 protein in skin specimens of cGVHD mice increased to 7.17 on the 96th day after transplantation, significantly higher than 4.36 in the hydrogen group.
Results
Conclusion: In this study, we found that oral hydrogen-rich water improved the survival rate and clinical symptoms of cGVHD mice by antioxidant and antiapoptosis. This study would pave the way for further clinical study, which may provide a new treatment option for cGVHD.