General Research
Model: rat
Hydrogen-rich saline protects rat from oxygen glucose deprivation and reperusion-induced apoptosis through VDAC1 via Bcl-2
Simplified Version Available
How Hydrogen-Rich Saline Helps Protect Against Stroke Damage
A recent study found that hydrogen-rich saline may help protect against stroke damage by reducing cell death and protecting brain cells. The solution works by affecting a protein called VDAC1, which helps regulate the balance of ions in the cell. This could potentially lead to new treatments for stroke, which is a major cause of disability and death worldwide.
Read Simplified ArticleAbstract
Publish Year 2019 Country China Rank Positive Journal Brain Research Primary Topic Whole Body Secondary TopicStroke Model Cell Culture Tertiary TopicIschemia-Reperfusion Injury Vehicle Saline (Dissolved) pH Neutral Application Culture Media Comparison Complement
Background
Methods: The mitochondrial functions before and after oxygen glucose deprivation and reperfusion (OGD/RP) were determined with corresponding dyes. The expression of Bcl-2, Bax, VDAC1, cytochrome c and caspase 9 was detected using qRT-PCR and Western Blotting method. Then Bcl-2 inhibitor, AB-199, was applied to investigate the role of Bcl-2 in OGD/RP-induced cell apoptosis. Finally, we manipulated the expression of VDAC1 with plasmids transfection to understand the effects of VDAC1 on Bcl-2-mediated anti-apoptosis in OGD/RP.
Methods
Results: In this study, we demonstrated that hydrogen-rich saline (HRS) reduced OGD/RP-mediated neuronal loss by stimulating the expression of Bcl-2, which suppressed the activity of VDAC1. Consequently, HRS maintained the mitochondrial functions, restrained the release of cytochrome c and caspase 9 activation, resulting in ameliorated cell viability. Conclusions: HRS ameliorated OGD/RP-induced PC-12 cell apoptosis and provided a novel treatment option for ischemia.