General Research
Model: pig
Hydrogen-rich saline attenuates eosinophil activation in a guinea pig model of allergic rhinitis via reducing oxidative stress
Simplified Version Available
How Hydrogen-Rich Saline Can Help Alleviate Allergic Rhinitis Symptoms
A study found that hydrogen-rich saline can help alleviate symptoms of allergic rhinitis by reducing oxidative stress and eosinophil activation. This natural and non-invasive treatment may be a promising new option for people who suffer from this common condition. More research is needed to confirm these findings in humans.
Read Simplified ArticleAbstract
Publish Year 2017 Country China Rank Positive Journal Journal of Inflammation Primary Topic Nose Secondary TopicRhinitis Model Guinea Pig Tertiary TopicInflammation Vehicle Saline (Dissolved) pH Neutral Application Injection Comparison Complement
Background
Methods: Thirty two guinea pigs were random divided into four groups, and AR model was established through ovalbumin sensitization. The guinea pigs were injected with hydrogen-rich saline (Normal-HRS and AR-HRS group) or normal saline (control and AR group). The frequencies of sneezing and scratching were recorded. The IgE level, blood eosinophil count and eosinophil cationic protein (ECP) level in serum were measured. The serum malondialdehyde (MDA) and superoxide dismutase (SOD) assays were also measured to evaluate oxidative stress. The expression levels of eotaxin mRNA and protein in the nasal mucosa were also determined by real-time RT-PCR, Western blot and immunofluorescence.
Methods
Results: HRS reduced the ROS and MDA levels and increased SOD level in guinea pigs of AR-HRS group accompanied with decreased frequency of sneezing and scratches. Meanwhile, there was a decline of the number of eosinophils cells in blood and of thelevel of ECP in serum in the AR-HRS group. HRS also significantly decreased the expression of eotaxin in nasal mucosa.
Results
Conclusion: HRS may play a protective role in attenuating allergic inflammation, and suppressing the increase and activation of eosinophils in AR possibly through antioxidation effect of hydrogen.