General Research
Model: mouse
Hydrogen Gas Therapy Attenuates Inflammatory Pathway Signaling in Septic Mice
Simplified Version Available
How Hydrogen Gas Therapy May Help Fight Sepsis and Inflammation
A recent study found that hydrogen gas therapy may help reduce inflammation in septic mice. This could have big implications for humans, as sepsis is a leading cause of death in hospitals. The study's findings suggest that hydrogen gas therapy may be a useful tool in fighting sepsis and other inflammatory conditions.
Read Simplified ArticleAbstract
Publish Year 2021 Country Japan Rank Positive Journal Journal of Surgical Research Primary Topic Whole Body Secondary TopicSepsis Model Mouse Tertiary TopicInflammation Vehicle Gas pH N/A Application Inhalation Comparison Complement
Background
Methods: Nine-week-old C57BL/6 male mice underwent cecal ligation and puncture (CLP) or sham procedure. Subsequently, the CLP model received immediate ± continuous inhalation of 7% H2. Mice were observed for a week to assess survival rates. Serum inflammatory cytokines were evaluated at 24 h after CLP procedure. Liver, intestine, and lungs in CLP mice receiving 24-h ± H2 therapy were assessed by RNA sequencing. Data were analyzed with Ingenuity Pathways Analysis (QIAGEN Inc).
Methods
Results: Seven-day survival rate in septic mice was significantly improved in the H2 inhalation group compared with that in the control group (75% versus 40%, P < 0.05). H2 treatment attenuated serum interleukin-6 and tumor necrosis factor-α levels at 24 h after CLP, and blood glucose levels were maintained in the H2-treated group. In RNA sequencing, canonical pathway analysis revealed inactivity of various inflammatory signaling pathways, for example, acute phase response signaling and STAT3 pathways, in the liver and intestine in the CLP model after 24-h H2 inhalation. We detected significantly decreased expressions of upstream regulator genes such as the CD14 antigen gene in the liver and various cytokine receptor genes in the intestine and lungs in the H2-treated group. Conclusions: These findings may contribute to clarifying the mechanism of action of H2 therapy in sepsis.