General Research
Model: mouse
Hydrogen gas protects against ovariectomy-induced osteoporosis by inhibiting NF-κB activation
Simplified Version Available
How Hydrogen Gas May Help Prevent Osteoporosis in Menopausal Women
A 2019 study found that inhaling hydrogen gas may help prevent osteoporosis in menopausal women by reducing inflammation. The study used mice that had undergone ovariectomy to simulate menopause. Hydrogen gas may be a useful tool in managing osteoporosis, a condition that weakens bones and is common in menopausal women.
Read Simplified ArticleAbstract
Publish Year 2019 Country China Rank Positive Journal Menopause Primary Topic Bone Secondary TopicOsteoporosis Model Mouse Tertiary TopicOvariectomy Vehicle Gas pH N/A Application Inhalation Comparison Complement
Methods
Results: H2 did not have any measurable effect on the proliferation of Raw264.7 cells. The number of osteoclasts and size of resorption pits of RANKL+H2-treated cells were 3 to 4 times less than RANKL treated cells. The expression of osteoclast marker genes of RANKL+H2-treated cells was 30% to 60% lower than RANKL-treated cells (P < 0.05). H2 markedly inhibited RANKL-induced activation, nuclear translocation, and transcriptional activity of NF-κB (P < 0.05, RANKL+H2 vs RANKL). The amount and density of trabecular bone and bone mineral density of ovariectomized mice were significantly less than sham-operated mice (P < 0.05 OVX vs sham). The amount of trabecular bone and bone mineral density of OVX mice that inhaled H2 were more than 40% higher, whereas the levels of serum proinflammatory cytokine interleukin 1β, IL-6, and tumor necrosis factor-α were more than 50% lower than those of OVX mice (P < 0.05). Conclusions: These results demonstrated that H2 could be an effective therapeutic agent of postmenopausal osteoporosis.