General Research
Model: rat
Effects of hydrogen-rich saline on rats with acute carbon monoxide poisoning
Simplified Version Available
Hydrogen-Rich Saline: A Potential Lifesaver for Carbon Monoxide Poisoning
A 2013 study found that hydrogen-rich saline may help alleviate the effects of carbon monoxide poisoning in rats. The researchers believe that the hydrogen in the saline reduced oxidative stress, which can damage cells and tissues. This study provides hope for a new way to combat carbon monoxide poisoning, a deadly condition that can happen to anyone.
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Publish Year 2013 Country China Rank Positive Journal Journal of Emergency Medicine Primary Topic Brain Secondary TopicEnvironmental Pollution Model Rat Tertiary TopicCarbon Monoxide Poisoning Vehicle Saline (Dissolved) pH Neutral Application Injection Comparison Complement
Background
Methods: Male Sprague-Dawley rats were subjected to CO poisoning. H(2)-rich saline was administered by peritoneal injection (6 mL/kg/24 h). We used the Morris water maze and the open field test to determine cognitive function. After cognitive function studies, rats were decapitated and the levels of trace elements copper (Cu), zinc (Zn), and iron (Fe) in serum and brain were assessed by flame atomic absorption spectrometry. Necrosis, apoptosis, and autophagy of neurons were assessed by H-E staining and immunohistochemical staining in another group of rats.
Methods
Results: H(2)-rich saline treatment improved the cognitive deficits and reduced the degree of necrosis, apoptosis, and cell autophagy in rats. Additionally, H(2)-rich saline decreased the content of Fe in serum and brain in these rats, and increased the content of serum Cu related to free radical metabolism. Conclusions: H(2)-rich saline may effectively protect the brain from injury after acute CO poisoning. The mechanism of this protection may be related to lessening oxidative damage by affecting trace elements in vivo.