General Research
Model: rat
Sirtuin Type 1 Mediates the Retinal Protective Effect of Hydrogen-Rich Saline Against Light-Induced Damage in Rats
Simplified Version Available
Hydrogen-Rich Saline: A Potential Guardian for Your Eyes
Hydrogen-rich saline may protect our eyes from damage caused by intense light. A study on rats found that this solution reduced retinal damage and identified Sirtuin Type 1 as a key protein in its protective effects. This discovery could lead to new ways to safeguard human vision, especially for people who spend a lot of time outdoors.
Read Simplified ArticleAbstract
Publish Year 2015 Country China Rank Positive Journal Investigative Ophthalmology and Visual Science Primary Topic Eye Secondary TopicRadioprotection Model Rat Tertiary TopicLight Damage Vehicle Saline (Dissolved) pH Neutral Application Injection Comparison Complement
Methods
Results: In light-damaged retinas, 5 days of HRS treatment increased Sirt1 expression, mitigated a- and b-wave amplitude reduction, and decreased the reduction of outer nuclear cell layers. The Sirt1 activator resveratrol mimicked the effect of HRS in light-damaged retinas. This result supported our hypothesis that Sirt1 mediates the protective effect of HRS. Additionally, the retinal protective effect of HRS was inhibited by both the Sirt1 inhibitor EX-527 and Sirt1 targeted short interfering RNAs. Hydrogen-rich saline also increased B-cell lymphoma 2 (Bcl-2) expression and the activity of the antioxidant enzyme superoxide dismutase (SOD). Conversely, HRS decreased Bcl2-associated X protein expression, cleaved caspase-3, and oxidant-stress product malondialdehyde (MDA) in a Sirt1-dependent manner. Conclusions: Sirt1 mediates light-induced damage mitigation by HRS through inhibition of apoptosis and oxidant-stress.
Purpose
Methods: Rats were treated with HRS for 5 days after intense light exposure, and then ERGs were performed and retinas were collected to evaluate the effect of HRS on Sirt1 expression. The necessity of Sirt1 for the retinal protective effect of HRS was investigated using the Sirt1 activator resveratrol, the Sirt1 inhibitor EX-527, and short interfering RNAs.