General Research
Model: in_vitro
RNA sequencing analysis reveals apoptosis induction by hydrogen treatment in endometrial cancer via TNF and NF-κB pathways
Simplified Version Available
Hydrogen Water and Cancer: A Promising New Study
A recent study found that hydrogen water can induce apoptosis in endometrial cancer cells by activating certain pathways in the body. The results are promising and suggest that hydrogen water could be a potential treatment for endometrial cancer. More research is needed, but the findings are exciting and could lead to new treatments for cancer.
Read Simplified ArticleAbstract
Publish Year 2020 Country China Rank Positive Journal Translational Cancer Research Primary Topic Uterus Secondary TopicCancer Model Cell Culture Tertiary TopicOncogenesis Vehicle Water (Dissolved) pH Neutral Application Culture Media Comparison Complement
Background
Methods: Ishikawa, HEC1A and AN3CA cells were incubated in DMEM medium with or without hydrogen. RNA sequencing was used to explore the association of hydrogen treatment with signaling pathways and functional genes in endometrial cancer cells. The apoptotic rates of the three endometrial cancer cells were evaluated by fluorescein isothiocyanate (FITC) Annexin V and Annexin V-allophycocyanin (APC)/propidium iodide double staining.
Methods
Results: RNA sequencing analysis revealed that hydrogen induced TNF/NF-κB and apoptosis pathways in endometrial cancer cells. The gene sets between hydrogen treatment groups and non-treated groups were mapped in accordance with Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) terms. Hydrogen treatment significantly increased the apoptotic rates of endometrial cancer cells. Conclusions: Taken together, our data indicate that hydrogen can serve as a therapeutic target for endometrial cancer via TNF/NF-κB pathway and apoptosis induction.