General Research
Model: in_vitro
Quantitative proteomics reveals the mechanisms of hydrogen-conferred protection against hyperoxia-induced injury in type II alveolar epithelial cells
Simplified Version Available
How Hydrogen Helps Protect Lungs from Damage
Researchers found that hydrogen helps protect lung cells from damage caused by too much oxygen. This could lead to new treatments for people with lung diseases. The study used quantitative proteomics to understand how hydrogen affects proteins in lung cells.
Read Simplified ArticleAbstract
Publish Year 2018 Country China Rank Positive Journal Experimental Lung Research Primary Topic Lung Secondary TopicSurgery/Transplantation Model Cell Culture Tertiary TopicHyperoxia Vehicle Medium (Dissolved) pH Neutral Application Culture Media Comparison Complement
Methods
Results: Hydrogen plays a protective role in hyperoxia-induced damage in AECIIs, as evidenced by reduced apoptosis, increased viability and survival, improved morphology, and enhanced transdifferentiation of AECIIs into AECIs. A total of 5782 proteins were identified in our study, of which 162 were significantly altered in abundance after hyperoxia exposure, and 97 were significantly altered in abundance in response to hydrogen treatment. The Gene Ontology and KEGG enrichment analyses identified a large number of proteins and biological processes that may responsible for the protective effect of hydrogen, including VEGFA, PDGFB, IGFBP3, EDN1, NADPH oxidase, the coagulation cascade, etc. Conclusions: Molecular hydrogen protects AECIIs from hyperoxic injury by complex mechanisms involving a variety of proteins and biological processes, such as VEGFA, PDGFB, IGFBP3, EDN1, NADPH oxidase and the coagulation cascade. These findings suggest novel pathways that need to be investigated as possible therapeutic targets for hyperoxia-induced lung injury.