General Research
Model: mouse
Protective effects of hydrogen gas against sepsis-induced acute lung injury via regulation of mitochondrial function and dynamics
Simplified Version Available
How Hydrogen Gas Fights Lung Damage from Sepsis
A 2018 study found that hydrogen gas therapy can help protect against sepsis-induced acute lung injury by regulating mitochondrial function and dynamics. This promising development could lead to new treatments for sepsis patients. Hydrogen gas therapy is a relatively simple and non-invasive treatment that could be used in conjunction with existing therapies to improve outcomes.
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Publish Year 2018 Country China Rank Positive Journal International Immunopharmacology Primary Topic Lung Secondary TopicSepsis Model Mouse Tertiary TopicLung Injury Vehicle Gas pH N/A Application Inhalation Comparison Complement
Background
Methods: By using cecal ligation and puncture (CLP), a classic septic model, we explored the role of 2% H2 treatment in sepsis-induced acute lung injury (ALI) linked to mitochondrial function and dynamics. We randomized male Institute for Cancer Research (ICR) mice into 4 groups: sham, sham + H2, CLP and CLP + H2. At 24 h after CLP or sham operations, we used histological examination and transmission electron microscopy (TEM) to observe lung slices. We analyzed oxygenation index (PaO2/FiO2), mitochondrial-membrane potential (MMP), adenosine triphosphate (ATP) levels, respiration control ratio (RCR) and mitochondrial-respiration complex activities (I and II) using commercial kits, and dynamin-related protein 1 (Drp1) and mitofusin-2 (MFN2) using Western blot.
Methods
Results: Therapy with 2% H2 increased PaO2/FiO2 ratios, MMP and ATP levels, RCR, complex I activity and MFN2 expression but decreased histological score and Drp1 levels in the presence of sepsis. These data indicated that inhalation of 2% H2 to regulate mitochondrial function and dynamics may be a promising therapeutic strategy for lung injuries induced by severe sepsis.