General Research
Model: dog
Protective effects of a hydrogen-rich preservation solution in a canine lung transplantation model
Simplified Version Available
How Hydrogen Therapy Could Revolutionize Lung Transplants
Researchers in Japan found that a hydrogen-rich preservation solution can help protect lungs during transplantation, reducing damage and improving outcomes. This breakthrough could make lung transplants safer and more successful. The study's findings have implications for people waiting for a lung transplant and could potentially impact other types of transplants and treatments.
Read Simplified ArticleAbstract
Publish Year 2020 Country Japan Rank Positive Journal Annals of Thoracic Surgery Primary Topic Lung Secondary TopicSurgery/Transplantation Model Dog Tertiary TopicTransplantation/Graft Injury Vehicle Solution (Dissolved) pH Neutral Application Immersion Comparison Complement
Background
Methods: Ten beagles underwent orthotopic left LTx after 23 hours of cold ischemia followed by reperfusion for 4 hours. Forty-five minutes after reperfusion, the right main pulmonary artery was clamped to evaluate the function of the implanted graft. The beagles were divided into two groups: control (CON group, n=5) and hydrogen (H2 group, n=5). In the CON group, the donor lungs were flushed and immersed during cold preservation at 4°C using ET-Kyoto solution, and in the H2 group, these were flushed and immersed using H2-rich ET-Kyoto solution. Physiological assessments were performed during reperfusion. After reperfusion, the wet-to-dry ratios were determined, and histological examinations were performed.
Methods
Results: Significantly higher partial pressure of arterial oxygen and significantly lower partial pressure of carbon dioxide were observed in the H2 group than in the CON group (p=0.045 and p<0.001, respectively). The wet-to-dry ratio was significantly lower in the H2 group than in the CON group (p=0.032). Moreover, in histological examination, less lung injury and fewer apoptotic cells were observed in the H2 group (p<0.001 and p<0.001, respectively). Conclusions: Our results demonstrated that the H2-rich preservation solution attenuated ischemia-reperfusion injury in a canine left LTx model. (247 words).