General Research Model: rat

Pretreatment with hydrogen-rich saline reduces the damage caused by glycerol-induced rhabdomyolysis and acute kidney injury in rats

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How Hydrogen-Rich Saline Can Protect Your Kidneys from Damage

Rhabdomyolysis is a condition where your muscles break down and release toxins into your bloodstream, damaging your kidneys. Researchers found that pretreating rats with hydrogen-rich saline reduced kidney damage and toxin levels. Hydrogen-rich saline works by destroying free radicals, which are harmful molecules that can damage your cells.

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Abstract

Publish Year 2014 Country China Rank Positive Journal Journal of Surgical Research Primary Topic Kidney Secondary TopicAcute Kidney Injury Model Rat Tertiary TopicRhabdomyolysis Vehicle Saline (Dissolved) pH Neutral Application Injection Comparison Complement

Background

Materials and methods: Forty-eight rats were randomly divided into four equal groups. Group 1 served as the control, group 2 was given 50% glycerol (10 mL/kg, intramuscular), group 3 was given glycerol after 7 d pretreatment with high dose HRS (10 mL/kg/d, intraperitoneal), and group 4 was given glycerol after 7 d pretreatment with low dose HRS (5 mL/kg/d, intraperitoneal). Renal health was monitored by serum creatinine (Cr), urea, and histologic analysis; rhabdomyolysis was monitored by creatine kinase (CK) levels; and oxidative stress was monitored by kidney tissue reactive oxygen species (ROS), malondialdehyde, 8-hydroxydeoxyguanosine (8-OH-dG), superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX) levels. Inflammation was monitored by interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) evaluation.