General Research
Model: human
Preconditioning with one-time hydrogen gas does not attenuate skin flap ischemia-reperfusion injury in rat models
Simplified Version Available
Can Hydrogen Gas Help Reduce Skin Damage During Surgery?
A recent study looked at whether hydrogen gas could help reduce skin damage during surgery, but found that a one-time treatment did not have a significant effect. Hydrogen gas has shown promise in other areas of research, and further studies may find a way to make it work for skin flap surgery. This area of research is important for finding new ways to protect our skin during medical procedures.
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Publish Year 2019 Country China Rank Neutral Journal Journal of Plastic Reconstructive and Aesthetic Surgery Primary Topic Skin Secondary TopicSurgery/Transplantation Model Rat Tertiary TopicPlastic Surgery Vehicle Gas pH N/A Application Inhalation Comparison Complement
Background
Methods: Forty-five adult male Sprague Dawley rats (body weight 220-250 g) were randomly divided into three groups: (1) Sham operation group (SH), (2) Ischemia-reperfusion injury group (IR), and (3) Ischemia-reperfusion injury with preconditioning hydrogen group (PRH). IR injury was induced by clamping the right superficial epigastric artery for 3 h. Before undergoing 3 h of IR management, the PRH group was treated with hydrogen inhalation for 1 h. On the third postoperative day, survival area and blood perfusion of the flap were assessed using laser Doppler flowmetry. RIP1 and RIP3 were examined by immunological detection and western blot analysis.
Methods
Results: Both the IR and PRH groups had less skin flap survival area and less blood perfusion than the sham group (P < 0.05). RIP1 and RIP3 were highly expressed in the IR and PRH groups when compared with those in the SH group (P < 0.05). There were no significant differences in flap survival rate (32.34 ± 2.19% and 33.09 ± 1.64%), average blood perfusion (41.66 ± 3.53 pu, 48.57 ± 2.83 pu), and expression of RIP1 and RIP3 (0.5167 ± 0.1409 and 0.4693 ± 0.1454) between the IR and PRH groups. Conclusions: Preconditioning with one-time inhaled hydrogen does not attenuate skin flap IR injuries in rat models.