Pharmacological post-conditioning with lactic acid and saturated hydrogen saline could attenuate myocardial apoptosis

Results: The pH of blood from right atrium after 3 min of reperfusion in Lac + Hyd group was significantly lower than that in R/I group (7. 32 ±0. 06 vs. 7. 43 ±0. 03, P <0. 05), the content of MDA was lower (1. 14 ± 0.16 vs. 1. 56 ± 0. 21, P < 0. 05) and the content of SOD was higher in Lac + Hyd group than those in R/I group (57. 92 ± 15. 12 vs. 35. 48 ± 12. 46, P < 0. 05). Apoptotic index of Lac + Hyd group was much lower than that of R/I group (9. 50 ± 1. 51) % vs. (15. 21 ± 1.91)%, P<0.05. After 30 min of reperfusion, the level of P-p38 in ischemic myocardia in Lac + Hyd group was significantly lower than that in R/I group (0. 46 ±0. 06 vs. 2. 18 ±0. 32, P <0. 05), the levels of P-JNK (0.59 ±0.03 vs. 1.62 ±0.29, P<0.05), TNFa (0.34 ±0.08 vs. 1.78 ±0.31, P<0.05) and Caspase-8 (0. 31 ±0. 07 vs. 1. 52 ± 0. 28, P 0. 05). After 30 min of reperfusion, there was no significant difference in the level of P-ERK between Lac + Hyd group and R/I group (0. 55 ± 0. 13 vs. 0. 57 ± 0. 05, P > 0. 05), and the level of P-ERK in Hyd group was significantly lower than that in R/I group (0. 30 ± 0. 09 vs. 0. 57 ± 0. 05, P < 0. 05), And the level of P-ERK in Lac Hyd and Lac + Hyd groups was significantly lower than that in M-Post group (1.21 ± 0.13, 0. 30 ± 0.09, 0. 55 ± 0.13 vs. 1. 96 ± 0. 39, P < 0.05).

Conclusion: Pharmacological post-conditioning with lactic acid and saturated hydrogen saline could be used instead of mechanical post-conditioning to inhibit the phosphorylation of p38/JNK and ERK, attenuating myocardial cell apoptosis.