General Research
Model: human
Lung Inflation With Hydrogen During the Cold Ischemia Phase Alleviates Lung Ischemia-Reperfusion Injury by Inhibiting Pyroptosis in Rats
Simplified Version Available
Breathe Easy: How Hydrogen Therapy May Revolutionize Lung Transplantation
Researchers in China found that using hydrogen gas during lung transplantation can help alleviate ischemia-reperfusion injury, a common complication of the procedure. The study, which used rats, found that hydrogen gas reduced tissue damage by inhibiting pyroptosis, a type of cell death. This discovery has promising implications for human lung transplantation and highlights the potential benefits of hydrogen therapy.
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Publish Year 2021 Country China Rank Positive Journal Frontiers in Physiology Primary Topic Lung Secondary TopicSurgery/Transplantation Model Rat Tertiary TopicTransplantation/Graft Injury Vehicle Gas pH N/A Application Ventilation Comparison Complement
Background
Methods: Adult male Wistar rats were randomly divided into the sham group, the control group, the oxygen (O2) group, and the hydrogen (H2) group. The sham group underwent thoracotomy but no lung transplantation. In the control group, the donor lungs were deflated for 2 h. In the O2 and H2 groups, the donor lungs were inflated with 40% O2 + 60% N2 and 3% H2 + 40% O2 + 57% N2, respectively, at 10 ml/kg, and the gas was replaced every 20 min during the cold ischemia phase for 2 h. Two hours after orthotopic lung transplantation, the recipients were euthanized.
Methods
Results: Compared with the control group, the O2 and H2 groups improved oxygenation indices, decreases the inflammatory response and oxidative stress, reduced lung injury, and improved pressure-volume (P-V) curves. H2 had a better protective effect than O2. Furthermore, the levels of the pyroptosis-related proteins selective nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3), cysteinyl aspartate specific proteinase (caspase)-1 p20, and the N-terminal of gasdermin D (GSDMD-N) were decreased in the H2 group.
Results
Conclusion: Lung inflation with 3% hydrogen during the cold ischemia phase inhibited the inflammatory response, oxidative stress, and pyroptosis and improved the function of the graft. Inhibiting reactive oxygen species (ROS) production may be the main mechanism of the antipyroptotic effect of hydrogen.