General Research
Model: rat
Hypothermic Machine Perfusion with Hydrogen Gas Reduces Focal Injury in Rat Livers but Fails to Restore Organ Function
Simplified Version Available
Hydrogen Gas: A Promising Tool for Liver Transplantation
Researchers in Japan found that using hydrogen gas during liver transplantation can reduce damage to the liver. This study shows promise for improving outcomes for people receiving liver transplants. Hydrogen gas is also being explored for its potential benefits in other areas of health, such as heart health and lung disease treatment.
Read Simplified ArticleAbstract
Publish Year 2023 Country Japan Rank Positive Journal Transplantation Proceedings Primary Topic Liver Secondary TopicSurgery/Transplantation Model Rat Tertiary TopicIschemia-Reperfusion Injury Vehicle Perfusion Solution (Dissolved) pH Neutral Application Perfusion Comparison Complement
Background
Methods: Liver grafts were procured from rats after 30 min of cardiopulmonary arrest. The graft was subjected to HMP for 3 hours at 7°C using Belzer MPS with or without dissolved H2 gas. The graft was reperfused using an isolated perfused rat liver apparatus at 37°C for 90 minutes. Perfusion kinetics, liver damage, function, apoptosis, and ultrastructure were evaluated.
Methods
Results: Portal venous resistance, bile production, and oxygen consumption rates were identical in the CS, MP, and MP-H2 groups. Liver enzyme leakage was suppressed by MP (vs control), whereas H2 treatment did not show a combination effect. Histopathology revealed poorly stained areas with a structural deformity just below the liver surface in the CS and MP groups, whereas these findings disappeared in the MP-H2 group. The apoptotic index in the CS and MP groups was high but decreased in the MP-H2 group. Mitochondrial cristae were damaged in the CS group but preserved in the MP and MP-H2 groups. Conclusions: In conclusion, HMP and H2 gas treatment are partly effective in DCD rat livers but insufficient. Hypothermic machine perfusion can improve focal microcirculation and preserve mitochondrial ultrastructure.