General Research
Model: in_vitro
Hydrogen treatment prevents lipopolysaccharide-induced pulmonary endothelial cell dysfunction through RhoA inhibition
Simplified Version Available
How Hydrogen Therapy May Help Prevent Lung Damage
Hydrogen therapy may help prevent lung damage in cases of sepsis or lung injury by reducing inflammation and preventing damage to lung cells. A recent study found that hydrogen gas can inhibit the activity of a protein involved in inflammation, reducing damage to lung cells. This promising treatment could have a significant impact on public health, especially since it's relatively non-invasive and low-risk.
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Publish Year 2020 Country China Rank Positive Journal Biochemical and Biophysical Research Communications Primary Topic Lung Secondary TopicSepsis Model Cell Culture Tertiary TopicLung Injury Vehicle Medium (Dissolved) pH Neutral Application Culture Media Comparison Complement
Background
Methods: We investigated the role of hydrogen-rich medium on regulating intercellular junction proteins under lipopolysaccharide (LPS) treatment which mimicked sepsis in vitro. Changes of cytoskeleton regulatory protein ROCK and RhoA as well as PMVEC apoptotic rate were examined.
Methods
Results: LPS treatment reduced the expression levels of occludin and VE-cadherin in PMVECs, while hydrogen-rich medium can recover these changes. Furthermore, H2 can significantly ameliorate the excessive expression of ROCK and RhoA under sepsis-mimicking condition. The application of RhoA activator U-46619 resulted in a more significant elevation in cell apoptotic rate as well as reduction in the expression of junctional proteins. Using H2 can almost completely inhibit the effects of RhoA activator. Conclusions: Our findings suggest that RhoA is a crucial protein in the signaling pathway of LPS-induced endothelial cell dysfunction. Hydrogen treatment can prevent LPS-induced junctional injury and cell death by inhibiting the activity of RhoA.