Hydrogen Water
General Research Model: rat

Hydrogen-rich water treatment targets RT1-Db1 and RT1-Bb to alleviate premature ovarian failure in rats

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Can Hydrogen-Rich Water Help Women with Premature Ovarian Failure?

A recent study found that hydrogen-rich water treatment can help alleviate premature ovarian failure in rats by reducing oxidative stress and inflammation. The findings are promising for women with this condition and suggest that hydrogen-rich water may be a potential complementary therapy. More research is needed to confirm the results in humans.

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Abstract

Publish Year 2023 Country China Rank Positive Journal PeerJ Primary Topic Ovaries Secondary TopicPremature Ovarian Failure Model Rat Tertiary TopicOxidative Stress Vehicle Water (Electrolysis) pH Neutral Application Ingestion Comparison Complement

Background

Methods: Based on cyclophosphamide (CTX)-induced POF rat models, the protective role of HRW treatment was mainly determined through serum 17-β-estradiol (E2), follicle-stimulating hormone (FSH), anti-mullerian hormone (AMH) levels, ovarian histomorphological analysis, and TUNEL assay. Tandem mass tag (TMT)-based quantitative proteomic analysis was then conducted on ovarian tissues, and the targets of HRW in POF were identified integrating differential expression analysis, functional enrichment analysis, and interaction analysis.

Methods

Results: In HRW treatment of POF rats, the serum AMH and E2 levels significantly increased, and FSH level significantly reduced, indicating the protective role of HRW. After TMT quantitative proteomic analysis, a total of 16 candidate differentially expressed proteins (DEPs) were identified after the cross analysis of DEPs from POF vs. control and POF+HRW vs. POF groups, which were found to be significantly enriched in 296 GO terms and 36 KEGG pathways. The crucial targets, RT1-Db1 and RT1-Bb, were finally identified based on both protein-protein interaction network and GeneMANIA network. Conclusions: The HRW treatment could significantly alleviate the ovarian injury of POF rats; RT1-Db1 and RT1-Bb are identified as two crucial targets of HRW treatment in POF rats.