General Research
Model: rat
Hydrogen-rich saline regulates the polarization and apoptosis of alveolar macrophages and attenuates lung injury via suppression of autophagy in septic rats
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How Hydrogen-Rich Saline May Help Reduce Lung Injury
A recent study found that hydrogen-rich saline may help reduce lung injury in septic rats by regulating immune cells and reducing self-destruction. This could lead to new treatments for serious conditions like sepsis and lung injury. Hydrogen therapy has shown promise in treating various lung diseases, and this study adds to the growing evidence.
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Publish Year 2021 Country China Rank Positive Journal Annals of Translational Medicine Primary Topic Lung Secondary TopicSepsis Model Rat Tertiary TopicLung Injury Vehicle Saline (Dissolved) pH Neutral Application Injection Comparison Complement
Background
Methods: Male Sprague-Dawley (SD) rats were used to prepare the sepsis-induced lung injury animal model. Rat lung tissue was harvested after lipopolysaccharide (LPS) treatment, in the presence or absence of HRS, and the AMs were analyzed for changes in polarization, apoptosis, and autophagy. The rat AM cell line NR8383 was used to examine these processes in vitro using Western blot analysis, flow cytometry, and transmission electron microscopy.
Methods
Results: LPS-induced ALI in rats was associated with an increase in autophagy, apoptosis, and M1 polarization but a decrease in M2 polarization in AMs. These effects were reversed by administration of HRS. Inhibition of AM autophagy with 3-methyladenine (3-MA) decreased apoptosis and M1 polarization and increased M2 polarization, paralleling the effects of HRS. Conclusions: HRS could attenuate ALI in septic rats through regulation of AM polarization and a reduction in apoptosis by suppressing autophagy. This may represent a potential novel therapeutic target for the treatment of ALI caused by sepsis.