General Research
Model: rat
Hydrogen-rich saline reduces tissue injury and improves skin flap survival on a rat hindlimb degloving injury model
Simplified Version Available
Healing Wounds with Hydrogen: How a Simple Solution Can Improve Skin Health
A study found that hydrogen-rich saline can reduce tissue injury and improve skin flap survival in rats with degloving injuries. This breakthrough has significant implications for human wound healing and could potentially be used to improve skin health. Hydrogen-rich saline is a simple and non-invasive treatment that promotes healing by neutralizing free radicals and reducing inflammation.
Read Simplified ArticleAbstract
Publish Year 2020 Country Turkey Rank Positive Journal Journal of Plastic, Reconstructive, and Aesthetic Surgery Primary Topic Skin Secondary TopicSurgery/Transplantation Model Rat Tertiary TopicPlastic Surgery Vehicle Saline (Dissolved) pH Neutral Application Injection Comparison Complement
Background
Methods: Twenty-one Sprague-Dawley rats were divided into three groups (control, saline and HRS). Degloving injury model was established, and flaps were sutured back following 5 min of ischemia. The control group did not receive any treatment. The saline group received intraperitoneal physiological saline (10 ml/kg) and the HRS group received intraperitoneal HRS solution (10 ml/kg) postoperatively and daily for 5 days after the operation. Skin samples were obtained for histological, immunohistochemical and biochemical evaluations.
Methods
Results: Inflammation was lower in the HRS compared with saline (p = 0.02) and control (p = 0.004) groups. Edema was lower in the HRS compared with saline (p = 0.02) and control (p = 0.001) groups. Malondialdehyde (MDA) level was lower in the HRS than the control group (p = 0.01). Total antioxidant level was higher in the HRS compared with saline (p = 0.009) and control (p = 0.03) groups. Total oxidant level was lower in the HRS than the control group (p = 0.02). Oxidative stress index was lower in the HRS compared with saline (p = 0.001) and control (p = 0.0001) groups'. Vascular proliferation was higher in the HRS compared with the control group (p = 0.01).
Results
Conclusion: Repeated HRS injections after trauma increased the viability of skin flap in rat degloving injury model by decreasing local tissue injury, due to its antioxidant, anti-inflammatory and angiogenic effects.