General Research
Model: rat
Hydrogen-rich saline prevents neointima formation after carotid balloon injury by suppressing ROS and the TNF-α/NF-κB pathway
Simplified Version Available
How Hydrogen-Rich Saline Can Help Prevent Heart Damage After Surgery
Hydrogen-rich saline may help prevent heart damage after surgery by reducing oxidative stress and inflammation. A study found that it suppressed the production of ROS and the TNF-α/NF-κB pathway, which can cause inflammation and damage. This discovery could lead to new treatments for heart surgery patients.
Read Simplified ArticleAbstract
Publish Year 2012 Country China Rank Positive Journal Atherosclerosis Primary Topic Heart Secondary TopicSurgery/Transplantation Model Rat Tertiary TopicAngioplasty Vehicle Saline (Dissolved) pH Neutral Application Injection Comparison Complement
Background
Methods: We assessed the influence of a hydrogen-rich saline solution (HRSS) by daily injection in rats. Rats were euthanized to evaluate the neointima. ROS, malondialdehyde (MDA) and superoxide dismutase (SOD) and reduced glutathione (GSH), were detected in the injured artery. Macrophage infiltration and the production of inflammatory factors (i.e., IL-6, TNF-α and NF-κB) were also observed. The in vitro effects of hydrogen on vascular smooth muscle cell (VSMC) proliferation were also measured.
Methods
Results: HRSS decreased the neointima area significantly. The neointima/media ratio was also reduced by HRSS. There was a decline in the number of PCNA-positive cells in the intima treated with HRSS. Meanwhile, HRSS ameliorated the ROS and MDA levels and increased SOD, reduced GSH levels in the injured carotid. In addition, the levels of inflammatory factors, such as IL-6, TNF-α and NF-κB p65, were attenuated by HRSS. In vitro studies also confirmed the anti-proliferative capability of the hydrogen solution and ROS generation in VSMCs induced by PDGF-BB.
Results
Conclusion: HRSS may have a protective role in the prevention of neointima hyperplasia and restenosis after angioplasty. HRSS may partially exert its role by neutralizing the local ROS and suppressing the TNF-α/NF-κB pathway.