General Research
Model: rat
Hydrogen-Rich Saline Prevents Early Neurovascular Dysfunction Resulting from Inhibition of Oxidative Stress in STZ-Diabetic Rats
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How Hydrogen-Rich Saline May Help Prevent Diabetes-Related Vision Problems
A 2013 study found that hydrogen-rich saline may help prevent vision problems in people with diabetes by reducing oxidative stress and preventing blood vessel damage. This could lead to new treatments for diabetic retinopathy. The study used rats with diabetes and found promising results, which could translate to human treatments.
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Publish Year 2013 Country China Rank Positive Journal Current Eye Research Primary Topic Eye Secondary TopicDiabetes Model Rat Tertiary TopicDiabetic Retinopathy Vehicle Saline (Dissolved) pH Neutral Application Injection Comparison Complement
Purpose
Materials and methods: Male Sprague-Dawley rats with streptozotocin (STZ)-induced diabetes mellitus (DM) were injected intraperitoneally with 5 ml/kg hydrogen-saturated (experimental) or plain (control) saline daily for one month. Visual function and blood-retinal barrier (BRB) integrity were evaluated by electroretinography (ERG) and bovine serum albumin (BSA)-fluorescence, respectively. Histological changes in the inner retina were assessed by light microscopy. Biomarkers of oxidative stress, including 4-hydroxynonenal (4-HNE) and 8-hydroxy-2-deoxyguanosine (8-OH-dG), and antioxidant enzymes, including superoxide dismutase, glutathione peroxidase, glutathione reductase and glutathione transferase, were evaluated by ELISA. Synaptophysin and brain-derived neurotrophic factor (BDNF) levels were measured by immunoblotting.