General Research
Model: rat
Hydrogen Preconditioning During Ex Vivo Lung Perfusion Improves the Quality of Lung Grafts in Rats
Simplified Version Available
Revolutionizing Lung Transplants: How Hydrogen Can Improve Graft Quality
A recent study found that hydrogen preconditioning during ex vivo lung perfusion can improve the quality of lung grafts in rats. This technique has the potential to increase the success rate of lung transplants and could have implications for other types of transplants. By reducing inflammation and improving the overall health of the lungs, hydrogen preconditioning may lead to better outcomes for patients.
Read Simplified ArticleAbstract
Publish Year 2014 Country United States Rank Positive Journal Transplantation Primary Topic Lung Secondary TopicSurgery/Transplantation Model Rat Tertiary TopicTransplantation/Graft Injury Vehicle Gas pH N/A Application Ventilation Comparison Complement
Background
Methods: Rat heart-lung blocks were mounted on an acellular normothermic EVLP system for 4 hr and ventilated with air or air supplemented with 2% hydrogen. Arterial and airway pressures were monitored continuously; perfusate was sampled hourly to examine oxygenation. After EVLP, the lung grafts were transplanted orthotopically into syngeneic rats, and lung function was examined.
Methods
Results: Placing lung grafts on EVLP resulted in significant upregulation of the messenger RNAs for several proinflammatory cytokines, higher glucose consumption, and increased lactate production. Hydrogen administration attenuated proinflammatory changes during EVLP through upregulation of the heme oxygenase-1. Hydrogen administration also promoted mitochondrial biogenesis and significantly decreased lactate production. Additionally, in the hydrogen-treated lungs, the expression of hypoxia-inducible factor-1 was significantly attenuated during EVLP. These effects were maintained throughout EVLP and led to better posttransplant lung graft function in the recipients of hydrogen-treated lungs. Conclusions: Lung grafts on EVLP exhibited prominent proinflammatory changes and compromised metabolic profiles. Preconditioning lung grafts using inhaled hydrogen attenuated these proinflammatory changes, promoted mitochondrial biogenesis in the lungs throughout the procedure, and resulted in better posttransplant graft function.