General Research
Model: mouse
Hydrogen inhibits the proliferation and migration of gastric cancer cells by modulating lncRNA MALAT1/miR-124-3p/EZH2 axis
Simplified Version Available
Hydrogen Gas: A New Hope for Stomach Cancer Treatment
A new study from China suggests that inhaling hydrogen gas may help slow down the growth and spread of stomach cancer cells. The research found that hydrogen gas disrupts a complex process that helps cancer cells grow and multiply. This discovery could lead to new, more effective treatments for stomach cancer with fewer side effects.
Read Simplified ArticleAbstract
Publish Year 2021 Country China Rank Positive Journal Cancer Cell International Primary Topic Stomach Secondary TopicCancer Model Mouse Tertiary TopicNovel Therapy Vehicle Gas pH N/A Application Inhalation Comparison Complement
Background
Methods: Gastric cancer cell lines MGC-803 and BGC-823 were treated with or without H2 /O2 gas mixture (66.7%:33.3% v/v). Proliferation and migration were assessed by MTT and scratch wound healing assays respectively. The expression of lncRNA MALAT1, miR-124-3p, and EZH2 was analyzed by real-time quantitative PCR and/or western blot. Tumor growth was estimated using xenograft mouse model.
Methods
Results: H2 gas significantly inhibited gastric tumor growth in vivo and the proliferation, migration, and lncRNA MALAT1 and EZH2 expression of gastric cancer cells while upregulated miR-124-3p expression. LncRNA MALAT1 overexpression abolished all the aforementioned effects of H2. LncRNA MALAT1 and miR-124-3p reciprocally inhibited the expression of each other. MiR-124-3p mimics abrogated lncRNA MALAT1 promoted EZH2 expression and gastric cancer cell proliferation and migration. Conclusions: These data demonstrated that H2 might be developed as a therapeutics of gastric cancer and lncRNA MALAT1/miR-124-3p/EZH2 axis could be a target for intervention.