General Research
Model: rabbit
Effects of intraperitoneal hydrogen injection on nitric oxide synthase mRNA and malondialdehyde following limb ischemia-reperfusion in rabbits
Simplified Version Available
How Hydrogen Therapy May Help Reduce Tissue Damage After Surgery
Hydrogen therapy may help reduce tissue damage after surgery by increasing the production of nitric oxide synthase mRNA and reducing oxidative stress. A study in rabbits found that injecting hydrogen gas into the body reduced tissue damage after limb ischemia-reperfusion. This could lead to faster recovery times and fewer complications for people undergoing surgery.
Read Simplified ArticleAbstract
Publish Year 2015 Country China Rank Positive Journal Acta Orthopaedica et Traumatologica Turcica Primary Topic Muscle Secondary TopicSurgery/Transplantation Model Rabbit Tertiary TopicIschemia-Reperfusion Injury Vehicle Gas pH N/A Application Injection Comparison Complement
Methods
Results: Both iNOS mRNA expression and serum MDA levels were higher in the I/R group than the Sham group (P < 0.01) and lower in the IRH group than the I/R group (P < 0.01 or P < 0.05) at various time points after reperfusion. The eNOS mRNA expression level exhibited no significant difference between the I/R and Sham groups following reperfusion but was significantly higher in the IRH group than in the Sham group (P < 0.01 or P < 0.05). Conclusions: During the I/R process, the expression of iNOS mRNA was up-regulated along with an increase in MDA. Intraperitoneal injection of H2 could down-regulate iNOS mRNA expression and up-regulate eNOS mRNA expression in the I/R process, suggesting a protective effect of H2 in I/R-induced skeletal muscle injury.