General Research Model: rat

Effects of hydrogen-rich saline in neuroinflammation and mitochondrial dysfunction in rat model of sepsis-associated encephalopathy

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Hydrogen Therapy: A Promising Treatment for Brain Inflammation

A recent study found that hydrogen-rich saline reduced brain inflammation and improved outcomes in rats with sepsis-associated encephalopathy. This has implications for human health and suggests that hydrogen therapy could be a promising treatment for conditions characterized by brain inflammation. More research is needed, but the findings are encouraging.

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Abstract

Publish Year 2022 Country China Rank Positive Journal Journal of Translational Medicine Primary Topic Brain Secondary TopicSepsis Model Rat Tertiary TopicEncephalopathy Vehicle Saline (Dissolved) pH Neutral Application Injection Comparison Complement

Background

Materials and methods: Rats were challenged with lipopolysaccharide (LPS) at a dose of 8 mg/kg injected intraperitoneally to induce sepsis and hydrogen-rich saline (HRS) administered 1 h following LPS induction at a dose of 5 ml/kg. Rats were divided into: sham, sham + HRS, LPS and LPS + HRS. At 48 h, rats were sacrificed and Nissl staining for neuronal injury, TUNEL assay for apoptotic cells detection, immunohistochemistry, and ELISA protocol for inflammatory cytokines determination, mitochondrial dysfunction parameters, electron microscopy and western blot analysis were studied to examine the effect of HRS in LPS-induced septic rats.

Results

Conclusion: These data demonstrated that HRS can improve survival rate, attenuate neuroinflammation, astrocyte and microglial activation, neuronal injury and mitochondrial dysfunction in juvenile SAE rat model, making it a potential therapeutic candidate in treating paediatric SAE. Keywords: Apoptosis; Astrocyte; Microglia; Mitochondrial dysfunction; Neuroinflammation; Neuronal injury; Paediatrics; Sepsis-associated encephalopathy.