General Research
Model: rat
Effect of Hydrogen on AM Pyroptosis Induced by Severe Burns in Rats
Simplified Version Available
Healing Burns with Hydrogen: A New Frontier in Wound Care
Hydrogen therapy is showing promise in helping our bodies heal from severe burns by reducing pyroptosis, a type of cell death that can lead to tissue damage and slower healing. A recent study on rats found that hydrogen gas inhalation reduced pyroptosis and promoted faster healing. This could be a new tool in the fight against burn injuries, reducing the risk of long-term damage and improving quality of life.
Read Simplified ArticleAbstract
Publish Year 2023 Country China Rank Positive Journal Journal of Personalized Medicine Primary Topic Skin Secondary TopicWound Healing Model Rat Tertiary TopicBurn Vehicle Gas pH N/A Application Inhalation Comparison Complement
Background
Methods: In this study, histological changes in rat lungs in vivo were evaluated by micro-CT, and histological changes in isolated lungs were evaluated by hematoxylin and eosin (HE) staining. The expressions of leucine rich repeat (LRR) and pyrin domain (PYD) containing protein 3 (NLRP3), caspase-1 and Gasdermin-D (GSDMD) were analyzed by Western blotting. The expression of GSDMD was measured by immunofluorescence to evaluate the levels of lung inflammation and pyroptosis. The level of inflammation was assessed by enzyme-linked immunosorbent assay (ELISA). Pyroptosis was observed by transmission electron microscopy.
Methods
Results: We observed that severe burn resulted in increased IL-1β and IL-18, overexpression of NLRP3 and caspase-1 proteins, and pyroptosis in rat lung tissues, as demonstrated by GSDMD overexpression and electron microscopy of AMs. We also observed that hydrogen treatment partially reversed the increase in lung tissue density and reduced pulmonary inflammation. Moreover, hydrogen reduced the HE pathological injury score in the lung tissues of severely burned rats. Hydrogen treatment significantly reduced the contents of IL-1β and IL-18 in the lung tissues and decreased the expression of NLRP3, caspase-1 and GSDMD proteins compared with the burn group. Transmission electron microscopy results also showed that the number of AM membrane pores was significantly reduced in the hydrogen treatment group. Conclusions: The results of this study suggest that hydrogen may protect against ALI induced by burn injury by inhibiting pyroptosis of macrophages via NLRP3.